Figure 10.

Immunofluorescence analyses of ARPE-19 cells silenced with siRNA against the human β₂ isoform. ARPE-19 cells cultured for 4 weeks were transfected with or without siRNA specific to the human β₂ isoform, as shown in Figure 8. Confocal images of silenced (right panels) or not-silenced (left panels) cells immunostained for specific α and β isoforms are displayed. Apical expression of the β₂ and α₂ isoforms in mock-transfected cells is apparent in (A,C). Mislocalization and reductions in the fluorescence intensity of both β₂ and α₂ subunits are observed in silenced cells (B,D). Basolateral and apical expression of the β₁ isoform (E) is generally maintained in β₂-silenced cells. However, in the presented field, β₁ has a noted apical distribution pattern (F). The mainly basolateral pattern of the α₁ isoform (G,H) is maintained in silenced cells. Scale bar: 10 μm.