Figure 7.

The β₂ subunit in ARPE-19 cells does not stabilizes at the apical plasma membrane domain. Confocal images of the β₂ and α₂ subunits (in red) were analyzed for co-localization with CD147 (green), an apical marker (A,B). Co-localization of the basolateral markers β-catenin and N-cadherin (in red) with the β₂ subunits (in green) are shown in (C,D). Although an apparently apical pattern is observed for the Na⁺, K⁺-ATPase subunits β₂ and α₂, they do not co-localize with either apical or lateral markers. Scale bar: 10 μm. Representative Western blot of six independent experiments conducted in ARPE-19 cells for steady state surface labeling with biotin is presented in (E). Notice the lack of a β₂ subunit band in the biotin labeled lane.