Figure 9.

siRNA silencing of the human Na⁺, K⁺-ATPase β₂ isoform in mature ARPE-19 cells. ARPE-19 cells cultured for 4 weeks with ITS were knocked down using siRNAs against the human β₂ subunit. (A) mRNA levels of the α₁, α₂, β₁, and β₂ subunits were assessed in three independent experiments in ARPE-19 cells transfected with β₂ siRNA or without any siRNA (Mock) via qPCR. The extent of β₂ mRNA silencing was ~60%. (B) Western blot and quantitative results from silenced ARPE-19 cells treated as in (A) are presented. The amount of protein corresponding to the β₂ subunit was normalized to the level detected in the mock-transfected cells. The extent of β₂ protein silencing was ~40%.