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. 2016 Aug 19;128(14):1829–1833. doi: 10.1182/blood-2015-10-676452

Figure 1.

Figure 1

Human cytokine KI mice support engraftment of human mobilized PB CD34+ cells. (A) Representative fluorescence-activated cell sorter (FACS) analysis of the frequency of mouse and human CD45+ cells in BM in newborn mice 14 weeks posttransplantation. Numbers besides gated areas indicate percentages of cells. (B) Engraftment of hCD45+ cells in BM of newborn mice 10 to 16 weeks posttransplantation. Left panel indicates percentage of hCD45+ cells among total CD45+ cells. Right panel indicates absolute number of hCD45+ cells per hind legs (2 femurs and tibias). Each symbol represents an individual mouse; bars indicate mean values; n = 11 (NSG), n = 3 (SRG), n = 22 (MSTRG), n = 4 (MISTRG). Conv, conventional; KI, human cytokine KI. *P < .05; ***P < .001 (1-way analysis of variance [ANOVA] Kruskal-Wallis test with Dunn’s multiple comparison test). (C) hCD45+ cell engraftment in BM of newborn mice from split, matched individual donors. Each symbol represents mean frequency of hCD45% cells among total CD45+ cells. Mean frequency of human CD45+ cells in Conv vs KI mice was significantly different. *P < .05 (Conv vs KI; paired t test). (D) Engraftment of hCD45+ cells in blood (left) and spleen (right) 10 to 16 weeks posttransplantation. Each symbol represents an individual mouse; bars indicate mean values; n = 11 (NSG), n = 3 (SRG), n = 22 (MSTRG), n = 4 (MISTRG). *P < .05; ***P < .001 (1-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison test). (E) Engraftment of hCD45+ cells in BM, blood, and spleen in adult mice 12 to 16 weeks after transplantation. Panel shows percentage of hCD45+ cells among total CD45+ cells in each organ. Each symbol represents an individual recipient engrafted with hCD45+ cells; bars indicate mean values; n = 2 (NSG), n = 4 (MISTRG). (F) Representative FACS plots for hCD34+CD38+ and hCD34+CD38− cells in newborn recipients gated on mCD45− Lineage (Lin)− hCD45+ cells. Numbers beside gate indicate percentages of cells. (G) Frequency (left 2 graphs) as well as absolute number (right 2 graphs) of hCD34+CD38+, hCD34+CD38− cells per hind legs. Each symbol represents an individual newborn recipient engrafted with hCD45+ cells; bars indicate mean values; n = 5 (Conv; 2 NSG and 3 SRG), n = 19 (KI; 15 MSTRG and 4 MISTRG). *P < .05 (Mann-Whitney U test).