Table 1.
List of potential AICD-interacting proteins identified by MALDI-MS
| Spot No. | Protein name | Swiss-Prot accession no. | MW (kDa)/pI | MASCOT score | Molecular function |
|---|---|---|---|---|---|
| 1 | HSPA8 | P11142 | 71.1/5.37 | 247 | Molecular chaperone |
| 2 | NEXN protein (fragment) | Q7Z2X0 | 26.6/8.97 | 93 | Regulation of cell migration |
| 3 | NPD011/NUDC | Q9Y266 | 38.0/5.71 | 75 | Neurogenesis & neuronal migration |
| 4 | Notch2NL | Q7Z3S9 | 26.0/5.22 | 122 | Involved in notch signaling pathway |
| 5 | Fibrillin-2 precursor | P35556 | 314.8/4.76 | 142 | Structural components of extracellular calcium-binding microfibril |
| 6 | CASP8AP2 (fragment) | Q5T791 | 220.3/6.08 | 91 | Involved in apoptotic signaling |
| 7 | Cystatin S precursor | P01036 | 16.2/4.95 | 106 | Strongly inhibits papain and ficin |
| 8 | APOA4 | P06727 | 22.0/9.34 | 201 | May have a role in VLDL secretion and catabolism |
| 9 | GRP78/HSPA5 | P11021 | 72.1/5.03 | 226 | Facilitates multimeric protein complex assembly inside ER |
| 10 | Selenium-binding protein | Q13228 | 52.3/6.13 | 136 | May be involved in intra-Golgi protein transport |
| 11 | Galectin-1 | P09382 | 14.6/5.33 | 83 | May regulate apoptosis, cell proliferation and differentiation |
| 12 | Vimentin | P08670 | 53.5/5.06 | 139 | Class III intermediate filaments |
| 13 | HSP90β | P14625 | 62.6/6.4 | 198 | Molecular chaperone, with ATPase activity |
| 14 | Kelch-like protein 6 | Q8WZ60 | 69.1/5.83 | 53 | B-lymphocyte antigen receptor signaling |
| 15 | Fibrinogen γ | P02679 | 51.0/5.68 | 100 | Monomers that polymerize into fibrin |
| 16 | Dynein, axonemal | Q0VDD8 | 76.0/6.01 | 70 | Microtubule-associated motor protein |
| 17 | Lamin B2 | Q03252 | 67.6/5.29 | 55 | Component of nuclear lamina |
| 18 | SH3TC1 | Q8TE82 | 146.8/5.85 | 17 | Exact function is not known |
| 19 | Laminin γ | Q9Y6N6 | 171.9/6.29 | 27 | Attachment, migration and organization of cells into tissues |
| 20 | GAB2 | Q9UQC2 | 74.4/8.54 | 66 | Adaptor for transmitting various signals |
Note: Molecular functions for the identified proteins were obtained from the Uniprot database. MW, molecular weight; SH3TC1, SH3 domain and tetra-tricopeptide repeats-containing protein 1.