Fig. 1. Pdzk1ip1-GFP is expressed in a fraction of adult bone marrow HSCs.

A. The expression of GFP within the total BM cells of adult Pdzk1ip1-GFP mice and the phenotype of gated GFP⁺ cells. The phenotypes of total or Lin⁻ cells from control non-transgenic BM are shown for comparison. Note that GFP⁺ Lin⁺ cells correspond to SSC^hi granulocytes and GFP⁺ Lin⁻ cells correspond to HSCs.

B. Representative histograms of GFP expression in HSCs (LSK CD48⁻ CD150⁺), MPPs (LSK CD48⁺ CD150⁻), MyPs (Lin⁻ Sca1⁻ cKit⁺ CD150⁻), and MEPs (Lin⁻ Sca1⁻ cKit⁺ CD150⁺) from Pdzk1ip1-GFP and control non-transgenic animals.

C. The fraction of GFP⁺ cells within the indicated BM populations (MkP defined as Lin⁻ Sca1⁻ cKit⁺ CD150⁺ CD41⁺; B, B220⁺; T, TCRβ⁺; Gran, Gr1⁺ CD11b⁺ SSC^hi). Bars represent medians of individual animals shown (n=13 for all cells except lymphocytes, for which n=5).

D. The expression of GFP in the HSC and progenitor (MPP1–4) populations defined according to (Cabezas-Wallscheid et al., 2014; Wilson et al., 2008) and the fraction of GFP⁺ cells within each population (bars represent medians of individual animals shown, n=7).

E. The expression of GFP in the HSC (HSC-1 and HSC-2) and progenitor (MPP and HPC-1) populations defined according to (Oguro et al., 2013). The expression of CD229 in the HPC-1 population is shown as a control (orange shadow histogram). Bars represent medians of individual animals shown (n=8).

F. Expression of surface markers in GFP⁺ and GFP⁻ HSCs. Control populations showing the expression range of each marker are shown as grey shadow histograms. Representative of 3 individual animals.

G. The fraction of GFP⁺ and GFP⁻ HSCs and progenitors (MPPs and MyPs) that incorporated BrdU after a 3-day pulse. Shown are values from 6 individual animals. Statistical significance: *p ≤ 0.05; **p ≤ 0.01.

See also Fig. S1.