Figure 3.
(a) Average RNA Yield (ng/mm2) on porous structured Ti (ps-Ti) vs tissue culture polystyrene (tc-PS) for a representative donor (AMSC 283). RNA yields were standardized relative to the respective 2D surface areas of standard 6-well tissue culture plate (951 mm2) and ps-Ti discs (491 mm2). mRNA yields increased considerably by day 14 on ps-Ti compared to tc-PS samples. (b) Cell cycle markers (CCNB2, HIST2H4). (c) ECM production by collagenous bone-related proteins (COL1A1, COL3A1).