Table 2.
Sequences for primers used in qPCR reactions
| Gene ID | Class / Biology | Forward Primer | Reverse Primer |
|---|---|---|---|
| ACAN | B / Cartilage & ECM | GTGCCTATCAGGACAAGGTCT | GATGCCTTTCACCACGACTTC |
| ACTA2 | B / Smooth muscle | AAAAGACAGCTACGTGGGTGA | GCCATGTTCTATCGGGTACTTC |
| ADIPOQ | B / Fat | AACATGCCCATTCGCTTTACC | TAGGCAAAGTAGTACAGCCCA |
| ALPL | B / Bone | ACTGGTACTCAGACAACGAGAT | ACGTCAATGTCCCTGATGTTATG |
| ATF1 | TF | CTGGAGTTTCTGCTGCTGTC | GGCAATGGCAATGTACTGTC |
| ATF4 | TF | ATGACCGAAATGAGCTTCCTG | CTGGAGAACCCATGAGGTTTG |
| BGN | B / ECM | GAGACCCTGAATGAACTCCACC | CTCCCGTTCTCGATCATCCTG |
| CCNB2 | B / Cell cycle | CCGACGGTGTCCAGTGATTT | TGTTGTTTTGGTGGGTTGAACT |
| CD248 | B / Cell surface | ATCGCAGCCAACTATCCAGAT | TTCCAGGCAAATGAGTGGTGG |
| CD44 | B / Cell surface | CTGCCGCTTTGCAGGTGTA | CATTGTGGGCAAGGTGCTATT |
| COL1A1 | B / Bone | GTAACAGCGGTGAACCTGG | CCTCGCTTTCCTTCCTCTCC |
| COL3A1 | B / Bone | TTGAAGGAGGATGTTCCCATCT | ACAGACACATATTTGGCATGGTT |
| DCN | B / Cartilage | ATGAAGGCCACTATCATCCTCC | GTCGCGGTCATCAGGAACTT |
| DLX3 | TF | TACCCTGCCCGAGTCTTCTG | GCCCGAGTAGTAATCGTGCTG |
| DLX5 | TF | AGCTACGCTAGCTCCCTACCACC | GGTTTGCCATTCACCATTCTCAC |
| EZH1 | ER / Me writer | GACATGCTATTGAAGGCAACA | GACACCATTCTCAGGGAACAT |
| EZH2 | ER / Me writer | GACGGCTTCCCAATAACAGTA | AGTCCCTGCTTCCCTATCACT |
| FOSL1 | TF | GCCGCCCTGTACCTTGTATCT | CAGTGCCTCAGGTTCAAGCA |
| GAPDH | Norm / Housekeeping | ATGTTCGTCATGGGTGTGAA | TGTGGTCATGAGTCCTTCCA |
| HIST2H4A | B / Cell cycle | AGCTGTCTATCGGGCTCCAG | CCTTTGCCTAAGCCTTTTCC |
| IBSP | B / Bone | GAACCTCGTGGGGACAATTAC | CATCATAGCCATCGTAGCCTTG |
| ID1 | TF | CTGCTCTACGACATGAACGG | GAAGGTCCCTGATGTAGTCGAT |
| ID3 | TF | GAGAGGCACTCAGCTTAGCC | TCCTTTTGTCGTTGGAGATGAC |
| KLF10 | TF | CTTCCGGGAACACCTGATTTT | GCAATGTGAGGTTTGGCAGTATC |
| MEF2C | TF | TCGTGGAGACGTTGAGAAAG | TTCTTGTTCAATGCGGAATC |
| MGP | B / ECM | TCCGAGAACGCTCTAAGCCT | GCAAAGTCTGTAGTCATCACAGG |
| MSX2 | TF | ATGGCTTCTCCGTCCAAAGG | TCGTCGGGCGAAAACAAGTC |
| NKX3-1 | TF | CCCACACTCAGGTGATCGAG | GAGCTGCTTTCGCTTAGTCTT |
| PRRX1 | TF / MSC phenotype | CAGGCGGATGAGAACGTGG | AAAAGCATCAGGATAGTGTGTCC |
| RUNX1 | TF | CTGCCCATCGCTTTCAAGGT | GCCGAGTAGTTTTCATCATTGCC |
| RUNX2 | TF | TGGTTACTGTCATGGCGGGTA | TCTCAGATCGTTGAACCTTGCTA |
| RUNX3 | TF | AGGCAATGACGAGAACTACTCC | CGAAGGTCGTTGAACCTGG |
| SATB2 | TF | GTGCCCGGATGGTTACACC | CTTCCTACAAATATGGCGGCAT |
| SOX4 | TF | AGCGACAAGATCCCTTTCATTC | CGGGGTAGTCAGCCATGTG |
| SOX9 | TF | TGTATCACTGAGTCATTTGCAGTGT | AAGGTCTGTCAGTGGGCTGAT |
| SP1 | TF | CAGGTGCAAACCAACAGATTA | GCTGGAGTAGGTTTGGCATAG |
| SUV39H1 | ER / Me writer & reader | CCTGCCCTCGGTATCTCTAAG | ATATCCACGCCATTTCACCAG |
| SUV39H2 | ER / Me writer & reader | TCTATGACAACAAGGGAATCACG | GAGACACATTGCCGTATCGAG |
| TRPS1 | TF / MSC phenotype | ATGACACTCCTGTTGGGTACT | CGTGCTGCTTGCCATAATGTT |
(B = biomarker; TF = transcription factor; ER = epigenetic regulator; Me = methylation; MSC = mesenchymal stromal/stem cell).