Figure 7.

Pressure-dependent increases in outflow require NOS3 activity. The requirement of NOS3 activity was tested using Nos3 mutant or L-NAME–treated wild-type eyes. (A) Flow-rate versus pressure graph obtained from L-NAME–treated and control eyes. (B) Flow-rate versus pressure graph obtained from Nos3^−/− and control eyes. Black diamonds represent control eyes and black triangles either L-NAME–treated eyes or Nos3^−/− eyes. The equations representing the trendline for the linear part of the curve (in [A] and [B]) were: Control, y = 0.0053x + 0.0254, R² = 0.9997; L-NAME, y = 0.0038x + 0.0382, R² = 0.99971; and Nos3^−/− y = 0.0034x + 0.0211, R² = 0.9976. C was different between groups: Control, C = 5.3 ± 1.4 nL/min/mm Hg (whole eye C = 11.6 ± 3.08 nL/min/mm Hg); L-NAME, C = 3.8 ± 0.7 nL/min/mm Hg (whole eye C = 8.36 ± 1.54 nL/min/mm Hg); Nos3^–/–, C = 3.4 ± 0.6 nL/min/mm Hg (whole eye C = 7.48 ± 1.32 nL/min/mm Hg). C = mean ± SD. SEM is shown for data points in the graph.