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. Author manuscript; available in PMC: 2016 Oct 7.
Published in final edited form as: Nat Commun. 2015 Jan 21;6:6002. doi: 10.1038/ncomms7002

Figure 6. DLAF results in end-to-end coverage of transcriptome.

Figure 6

(a) Percentage of genes covered at 5′ and 3′ ends. RNA-SeQC data are shown for the 2,500 middle-expressed genes in WT mES cells. Data are shown for 12.5 million randomly selected non-rRNA and non-mtRNA reads. Average of two biological replicates is shown and error-bars indicate the range of data. (b) Image from the UCSC genome browser of the Nanog locus. DLAF read_1 shows a distinct coverage at the annotated TSS (green border) and an internal CS (pink border). Remapping of DLAF read_2 after ΔT9 analysis identified the polyadenylation site (yellow border). Signals are normalized to the total number of non-rRNA and non-mtRNA reads from each library.