Figure 3.

R848 induces local antibody responses after mucosal or systemic immunization. Macaques were immunised intranasally (A, E, I) or intramuscularly (B, F, J) with 200 μg KLH in combination with 500 μg R848 (n = 4 per group). Immunizations were administered at weeks 0, 4 and 8. KLH specific ELISA were performed for IgG (A, B, C) and IgA (E, F, G) in nasal lavage and IgG in vaginal samples (I, J, K). Relative coefficients of excretion (RCE) compared to albumin in nasal and vaginal samples (D, H, L). Data is presented as individual animals. Pooled data for each route is presented for each sample (C, F, I), where each point represents mean of n = 4 animals.