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. 2015 Aug 11;11(12):2831–2838. doi: 10.1080/21645515.2015.1074363

Figure 1.

Figure 1.

Schematic structures of HA protein of H5N1 and construction and characterization of recombinant HA1-FdFc, HA1-Fc, HA1-Fd, and HA1-His proteins. (A) Schematic structures of HA protein of A/Anhui/1/2005 (H5N1 HA). The HA protein consists of signal peptide (SP, residues 1-12), HA1 (residues 13-325) and HA2 (residues 326-554) fragments. (B) Construction of HA1-FdFc, HA1-Fc, HA1-Fd, and HA1-His protein fragments. The four fragments were constructed by fusing HA1 fragment with Fd and Fc (HA1-FdFc), Fc (HA1-Fc), Fd (HA1-Fd), or His6, respectively. IL2ss, a signal peptide, induces expressed proteins to culture supernatants. The purified protein samples were detected by SDS-PAGE, following by staining with Coomassie Blue (C), or Western blot using HA1-specific HA-7 mAb (D).