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Mouse vaccination procedure and sample collection. Groups of mice were i.n. immunized with HA1-FdFc, HA1-Fc, HA1-Fd, and HA1-His protein, respectively, or PBS, in the presence of Poly (I:C) or CpG adjuvant, or without adjuvant. Mice were immunized 3 times at 3-week intervals. Ten days later, after the last vaccination, mouse sera and lung wash were collected to detect IgG, IgA, and neutralizing antibodies.
