Table 3.
Overrepresented GO biological process pathways in matched vs mismatched MLC a
| Pathways | Homo sapiens genes | Regulated genes twofold matched vs mismatched | Expected | P‐value |
|---|---|---|---|---|
| Immune system process | 2480 | 68 | 21.20 | 2.95E‐17 |
| Response to stimulus | 1767 | 53 | 15.11 | 3.44E‐14 |
| Immune response | 725 | 34 | 6.2 | 1.11E‐13 |
| Response to interferon‐gamma | 113 | 16 | 0.97 | 1.00E‐12 |
| Macrophage activation | 325 | 21 | 2.78 | 1.88E‐10 |
| Cytokine‐mediated signalling pathway | 371 | 19 | 3.17 | 1.13E‐7 |
| Unclassified | 6816 | 25 | 58.28 | 1.33E‐6 |
| Signal transduction | 4019 | 62 | 34.36 | 1.21E‐4 |
| Cell communication | 4224 | 64 | 36.12 | 1.37E‐4 |
| Cellular defence response | 496 | 16 | 4.24 | 1.15E‐3 |
| Cell–cell signalling | 1259 | 26 | 10.76 | 4.83E‐3 |
| Cellular process | 6072 | 77 | 51.92 | 6.42E‐3 |
| Cell surface receptor linked signal transduction | 2049 | 35 | 17.52 | 9.34E‐3 |
| JAK‐STAT cascade | 112 | 7 | 0.96 | 9.94E‐3 |
| JNK cascade | 155 | 8 | 1.33 | 1.12E‐2 |
| Negative regulation of apoptosis | 264 | 10 | 2.26 | 1.80E‐2 |
| MAPKKK cascade | 454 | 13 | 3.88 | 2.79E‐2 |
| Extracellular transport | 138 | 7 | 1.18 | 3.56E‐2 |
| Apoptosis | 978 | 20 | 8.36 | 4.97E‐2 |
GO, gene ontology; MLC, mixed lymphocyte culture; MAPKK, mitogen‐activated protein kinase kinase kinase.
a
Gene ontology biological process pathways identified to be significantly overrepresented within differentially regulated genes between matched and mismatched MLC. Analysis was performed using the statistical overrepresentation tool of PANTHER classification system. P values are corrected using Bonferroni's multiple testing correction and a cut‐off of P < 0.05.