Table 1.
List of primers used for PCR and sequencing
| Code | F/R | Sequence (5′–3′) | Annealing position |
|---|---|---|---|
| ycf3‐F1P* | F | CAAGCDYTAAATAATATWGCTG | ycf3 (319‐340) |
| atpB‐F1P* | F | GCWAAAGCNCATGGTGGTGT | atpB (535‐554) |
| atpB‐F1.2P* | F | AARGTMGTWGAYYTATTAGC | atpB (427‐446) |
| atpB‐F1.3P* | F | GTHMGHGCNATTGCNATGAGTGC | atpB (187‐209) |
| atpB‐F2P* | F | GCDGTRGAYCCHTTAGATTCA | atpB (1,057‐1,077) |
| atpB‐2R† | R | AGCTTGWACAAATCTAAAAATA | atpB (810‐789) |
| atpB‐R2P* | R | GCWATAATATCTTGTAATTC | atpB (1,184‐1,165) |
| atpB‐R2.2P* | R | TCKACHACTAADCGRTCTTC | atpB (1,232‐1,213) |
| atpB‐R3P* | R | CAYRTAYAAATCRTTTCCTTC | atpB (609‐589) |
| atpB‐R4P* | R | GCWGRKCKATGRATAGGTAA | atpB (374‐355) |
| atpB‐R1P* | R | TTTGCTTTAGMDATWGCTTC | atpB (1,430‐1,411) |
| psaA130F‡ | F | AACWACWACTTGGATTTGGAA | psaA (126‐146) |
| psaA‐P2§ | R | NCCWGACCAAGMWARACAAC | psaA (639‐620) |
| psaA‐P2.2* | R | TCDGCRTTYTGRAACCAYTC | psaA (578‐559) |
| psaA‐P3§ | R | GCTGGDTTRTARTCACGAACC | psaA (1,253‐1,233) |
| psaA‐P3.2* | R | GCWGGRTTATARTCRCGDACC | psaA (1,253‐1,233) |
| psaA970R‡ | R | GCYTCTARAATYTCTTTCA | psaA (977‐959) |
| psaA970R2* | R | TRCTATGDCCRATNCCCCAA | psaA (958‐939) |
| psaA1760R‡ | R | CCTCTWCCWGGWCCATCRCAWGG | psaA (1,736‐1,714) |
| psaA1760R2* | R | CCRTCACAHGGRAAWCGGAA | psaA (1,724‐1,705) |
| psaA‐F3P* | F | CWGCWGAYTTTATGGTTCAYC | psaA (1,583‐1,603) |
| psaA‐P4§ | R | NGATTCAATHARYTCTTGCC | psaA (2,094‐2,075) |
| psaA‐P5§ | F | CAYCATATHCATGCNTTYAC | psaA (1,600‐1,619) |
| psaA‐F1P* | F | ATGCAATCNGAYRTTTGGGG | psaA (1,834‐1,853) |
| psaB‐F1P* | F | ATGCAATCNGAYRTTTGGGG | psaB (21‐40) |
| psaB‐F3P* | F | WGATGCWCAYMGWCCMCCTG | psaB (915‐934) |
| psaB‐F3.2P* | F | GSTATHGGNCAYAAYATGAAAGA | psaB (886‐908) |
| psaB‐R2P* | R | KAWTGCATACATATGYTGAG | psaB (1,065‐1,046) |
| psaB‐R3P* | R | CAATGCCAATARAAHGTAACCC | psaB (1,787‐1,766) |
| psaB‐R3.2P* | R | CAATARAAHGTAACCCAWCC | psaB (1,781‐1,762) |
| psaB‐R1P* | R | TTCCAGCHGTTGAWGCWATA | psaB (2,194‐2,175) |
| psaB‐F4P* | F | CCWTATGCHTTYATGGCAAAAGAT | psaB (1,069‐1,092) |
| psbA‐F‡ | F | ATGACTGCTACTTTAGAAAGACG | psbA (1‐23) |
| psbA‐F1P* | F | ACCGTTTATACATYGGTTGG | psbA (77‐96) |
| psbA500F‡ | F | CTCTGATGGWATGCCWYTAGG | psbA (504‐524) |
| psbA600R‡ | R | CCAAATACACCAGCAACACC | psbA (620‐601) |
| psbA600R2* | R | AWACACCAGCAACACCAGCC | psbA (616‐597) |
| psbA‐R1P* | R | TACGYTCRTGCATTACTTCC | psbA (1,003‐984) |
| psbA‐R1‡ | R | GCTAAATCTARWGGGAAGTTGTG | psbA (1,031‐1,009) |
| psbC‐P1.2* | F | CCACGTGGAAACGCYCTTTA | psbC (33‐52) |
| psbC‐P1* | F | TAGCTCATGCAGGYWTAATGG | psbC (152‐172) |
| psbC‐P3* | R | CTTGCCAAGGTTGRATATCATT | psbC (1,168‐1,147) |
| psbC‐P3.2* | R | ATWCCTTTTTCRAARCCAGC | psbC (1,379‐1,360) |
| psbC‐P4* | F | GCGTGAYAAAAAYAAAATGAC | psbC (444‐464) |
| psbC‐P5* | R | AARAATGGRAAWGAYTCTTC | psbC (431‐412) |
| psbC‐P6* | F | GAAGCWTCTCAAKCDCARGC | psbC (925‐944) |
| rbcL‐P2¶ | F | GAWCGRACTCGAWTWAAAAGTG | rbcL (19‐40) |
| rbc‐R2.5** | R | CCTTCATAAACAACACG | rbcL (587–571) |
| Ral‐R952¶ | R | CATACGCATCCATTTACA | rbcL (969–952) |
| rbcL‐P5* | F | CWTAYYTAAAAACWTTCCAAG | rbcL (440‐460) |
| rbcL‐P4* | R | AGKTGRTGCATYTGRCCACA | rbcL (1,178–1,159) |
| rbcL‐Rh3†† | F | TTAAYTCTCARCCDTTYATGCG | rbcL (629–650) |
| rbcL‐P1¶ | F | GKGTWATTTGTAARTGGATGCG | rbcL (944–965) |
| rbcL‐P3** | F | CARTTYGGWGGWGGTACDATTGG | rbcL (1,210‐1,232) |
| rbcL‐P3.2* | F | GARGGTCCTGADATTYTACGT | rbcL (1,327‐1,347) |
| rbcL‐Pla1 | F | TATTCTCGTGCAGTTGGTAG | rbcL (778‐797) |
| rbcL‐Pla4 | F | ATTTACTAACRGCTTGTGAC | rbcL (227‐246) |
| rbcL‐Pla5 | R | AAAGTATTATAGAATCCTCG | rbcL (1,055‐1,036) |
| rbcS‐P1¶ | R | GGATCATCTGYCCATTCTACAC | rbcS (122–101) |
| GazF2‡‡ | F | CCAACCAYAAAGATATWGGTAC | cox1 (104‐125) |
| cox1‐P1.2* | F | GATHTTYTTTATGGTDATGCC | cox1 (276‐296) |
| cox1‐P2* | R | GGDATACGDCGHGGCATACC | cox1 (1,400‐1,381) |
| cox1‐P3* | R | CNGTAAACATRTGRTGVGCC | cox1 (961‐942) |
| cox1‐P4* | F | DGCRGCNTTTACNATGTTTG | cox1 (1,230‐1,249) |
| cox1‐P5* | R | TAATACCNCCRGCYAAAACWGG | cox1 (700‐679) |
| cox1‐P5.2* | R | GCHGTDATTAADACHGACCA | cox1 (656‐637) |
| trnI‐P1.2* | R | GCTTATCAGGCGTACACTCT | trnI (20‐39) |
| trnI‐P1* | R | TTGAACGAWCGVCTTTACGC | trnI (38‐57) |