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. 2015 Sep 28;46(6):676–682. doi: 10.1111/age.12334

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© 2015 The Authors. Animal Genetics published by John Wiley & Sons Ltd on behalf of Stichting International Foundation for Animal Genetics.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Sanger sequencing of gDNA and cDNA in solid and spotted buffaloes revealed a heterozygous nonsense mutation in exon 3 in MITF. (a) Wild‐type gDNA sequence in solid buffaloes. (b) A nonsense mutation (NM_001001150.2) causing a premature translational stop c.328C>T, p.Arg110* at genomic position chr22: 32 322 242 was identified in MITF exon 3 in spotted buffaloes. Exon–intron borders in exon 3 are shown. (c) Only wild‐type sequence could be detected at cDNA level in spotted buffaloes carrying the c.328C>T mutation, suggesting nonsense‐mediated decay where only the wild‐type mRNA exists.