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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Nat Immunol. 2016 Aug 15;17(10):1226–1234. doi: 10.1038/ni.3533

Figure 4. HA labeling of ABCs and ASCs in blood after influenza vaccination and infection.

Figure 4

PBMCs isolated from healthy adult volunteers prior to, and 7, 14, 30 and 90 days after immunization with the 2013/14 TIV. (a) A FACS plot gated on isotype-switched B cells showing HA-positive ASCs (CD20lo) and HA-positive ABCs (CD20hi). (b) Histogram plots showing expression of Pax5 and IRF4 in HA-positive ABCs, HA-positive ASCs, and naïve B cells. (c) A FACS plot (gated on isotype-switched B cells) showing the kinetics of HA-positive B cells after TIV immunization. (d) A FACS plot showing CD71 expression on HA-positive ASCs (red dots) and HA-positive ABCs/MBCs (blue dots) overlaid over naïve B cells (grey dots) (e) PBMCs isolated from an influenza-infected patient. FACS plots gated on isotype-switched B cells showing HA-positive ASCs (CD20lo) and HA-positive ABCs (CD20hi) at days 0, 4 and 27 after enrollment.