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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Enzyme Microb Technol. 2016 Jul 20;93-94:29–43. doi: 10.1016/j.enzmictec.2016.07.009

Figure 5.

Figure 5

Activation of recombinant MMPs. Panel A: Activation of enzymes according to manufacturer’s recommendations. MMP-3 was activated with 5 μg/mL chymotrypsin for 30 min at 37 °C, MMP-1 and MMP-8 were activated with APMA, and MMP-12 was self-activated in TSB overnight at 37 °C. MMP-9 was purchased in an activated form. Panel B: Activation of enzymes with optimized trypsin/chymotrypsin mixture over 30 min at 37 °C. Enzyme activity was tested with 5 μM Knight SSP over 30 min. Activation of MMPs the same day (Panel C) or frozen overnight at −20 °C (Panel D), 6 d at −80 °C (Panel E), or 16 d at −80 °C (Panel F) with optimized trypsin/chymotrypsin mixture.