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. 2016 Oct 6;167(2):471–483.e10. doi: 10.1016/j.cell.2016.09.003

Figure S2.

Figure S2

Late COX Biogenesis Factors Do Not Interact with Nascent Chain Fragments as well as Yeast Coa3, Related to Figure 3

(A and B) Longer exposures of experiments shown in Figures 3F and 3G.

(C) Yeast Coa3 does not interact with Cox1 nascent chain. [35S]methionine labeling of mitochondrial translation products was carried out in organello mitochondria. Mitochondrial translational products were labeled for 25 min. Samples were treated with 0.1 mM puromycin (Pur) for 15 min and mitochondria solubilized in digitonin buffer and subjected to immunoisolation using Coa3-specific antiserum. Samples were analyzed by SDS-PAGE and digital autoradiography or western blot. Total 10%, eluate 100%.