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. 2016 Oct 6;167(2):471–483.e10. doi: 10.1016/j.cell.2016.09.003

Figure S3.

Figure S3

Mitochondrial Translation Is Affected upon Reduction of C12ORF62 and MITRAC12, Related to Figure 5

(A) Downregulation of the m-AAA protease does not restore COX1 levels. AFG3L2 was depleted by siRNA application for 72 hr and COX1 quantified after SDS-PAGE and digital autoradiography (mean ± SEM; n = 3).

(B) Steady-state levels of mitochondrial proteins after siRNA-mediated downregulation of MITRAC12. HEK293T cells were treated with siRNA molecules against Mitrac12 for 72 hr followed by western blot analysis.

(C and D) COX1 synthesis and stability in C12ORF62- and MITRAC12-deficient cells. C12ORF62 and MITRAC12 were depleted from HEK293T cells for 72 hr followed by radiolabeling for 1 hr. The cells were either harvested (C) or after media replacement, chased for indicated time points (D). Samples were analyzed by SDS-PAGE and digital autoradiography.