Figure 4.

RECK is a direct target of miR-590-5p.

Notes: (A) A schematic of the putative binding sites of miR-590-5p in the 3′-UTR of RECK is presented. (B) Luciferase reporter assays. Cells were transfected with reporters containing the wild-type (RECK-WT) or mutant-type RECK-3′-UTR (RECK-Mut) after transfection with miR-590-5p or a control. (C) The levels of RECK protein in GC cells were inhibited by miR-590-5p overexpression. **P<0.01.

Abbreviations: GC, gastric cancer; 3′-UTR, 3′ untranslated region.