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. Author manuscript; available in PMC: 2017 Sep 16.
Published in final edited form as: ACS Chem Biol. 2016 Aug 5;11(9):2636–2641. doi: 10.1021/acschembio.6b00489

Figure 2.

Figure 2

(A) Reconstituted NOCAP synthase modules 4–8 and the trans-AT-TE proteins. Docking domains from the 6-deoxyerythronolide B synthase (DEBS) were fused onto the C-terminus of ACP6 and the N-termini of KS4 and KS7. PKS abbreviations are as in Figure 1. (B) Stable isotope 13C-labeled malonyl-CoA was generated in situ enzymatically using MatB, S. coelicolor malonyl-CoA synthetase.