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. 2016 Sep 30;6:34478. doi: 10.1038/srep34478

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Copyright © 2016, The Author(s)

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Log curves for quantitation of the qRT-PCR analysis of ISC-hpNSC with dotted squares indicating the groupings of each sample run in triplicate. ISC-hpNSC and human fibroblasts group with the same level of POU5F1 expression of 10 hpSC. The negative control (NC) samples are below the 1 hpSC (10⁰ hpSC) cell reaction. Linearity of curves Ct was between 17–35 cycles.