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| Cell Population | Medium | Growth after 1 week | OCT-4 Expression |
|---|---|---|---|
| 0.1% hpSC in ISC-hpNSC | E8 medium | Colonies detected | Positive |
| 0.01% hpSC in ISC-hpNSC | E8 medium | Colonies detected | Positive |
| 0.001% hpSC in ISC-hpNSC | E8 medium | No colonies detected | Small signal detected |
| 100% ISC-hpNSC | E8 medium | No colonies detected | Negative |
| 100% hpSC | E8 medium | Confluent in 4 days | Positive |
| 100% hpSC | StemPro NSC medium | Significantly reduced cell numbers | Positive |
