Figure S3.

Reduced TORC1 Activity Produces a Specific and Informative Amino Acid Profile, Related to Figure 4

The application of sublethal rapamycin concentration (50 nM) generates a strong response on all amino acid concentrations. Alanine, aspartate, asparagine, glutamate, glutamine, branched chain amino acids and aromatic amino acids were substantially elevated whereas glycine, serine and lysine were greatly reduced and this response was strikingly similar to a deletion of the TORC1 component TCO89. All strains were grown for 6 hr and collected in exponential phase. Amino acid concentrations were determined as described for untreated WT and tco89Δ (n = 3) and for treated WT (n = 6) and are represented as mean ± SD.