Figure 3.

The influence of ICV injection of corticotropin-releasing factor receptor 2 antagonist, astressin₂-B, on central urocortin 3 counteracting acyl ghrelin-induced hyperphagia in freely fed satiated rats.

Notes: After the injection of either astressin₂-B (20 nmol/rat) or saline, urocortin 3 (2 nmol/rat) or vehicle was injected immediately, then two variants of acyl-ghrelin were administrated. After ICV injection, cumulative food intake was measured at 1, 2, 4, 8, 12, and 24 hours, respectively. The results are shown in (A–F), respectively. Data are presented as mean ± SEM. ^*P<0.05, compared between groups with and without urocortin 3 injection; ^#P<0.05, compared between groups with and without astressin₂-B injection; n=12 rats in each group.

Abbreviations: ICV, intracerebroventricular; SEM, standard error of the mean.