Figure S6.

Oculomotor and Trochlear, Serotonergic, and Dopaminergic neurons, Related to Figure 4 and 6

(A) Examples of genes regulated along pseudotime in mOMTN. No genes were significantly downregulated.

(B) Validation of the induction of Pvalb during maturation of mOMTN, shown alongside Isl1 in sagittal sections of mouse embryos (in situ hybridization data from the Allen Developing Mouse Brain Atlas) (scale bars 200 μm).

(C) Violin plots showing the expression of key genes involved in serotonergic synapse function, across all human cell types.

(D) Violin plots showing the expression of key genes involved in serotonergic synapse function, across all mouse cell types.

(E) Schematic of the function of genes in (C/D) in a serotonergic neuron, here drawn in place of their corresponding protein products (adapted from Deneris and Wyler, 2012).

(F) Spatial distribution of the five adult dopaminergic cell types (in situ hybridization data from Allen Mouse Brain Atlas) (scale bars 200 μm).

(G) Validation of AJAP1 as a pan-dopaminergic marker in the adult mouse brain (scale bar left 100 μm; scale bar right 20 μm).

(H) Scatterplots showing the level of expression of genes expressed above baseline in matching cell types (left) and the correlation of the cell types that express the genes at higher levels (right)