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Proinflammatory cytokines decreased the expression of RPE characteristic genes. Differentiated ARPE-19 cells were treated with interferon gamma (IFN-γ; 10 u/ml), tumor necrosis factor alpha (TNF-α; 1 ng/ml), interleukin-1 beta (IL-1β; ng/ml) either alone or in combination for 20 h in the absence of 1% serum. The gene transcripts were analyzed with real-time PCR. A: CDH1. B: RLBP1. C: RPE65. D: RDH5. *p<0.5 when compared to control, n = 4.
