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. 2016 Oct 8;22:1156–1168.

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Copyright © 2016 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Proinflammatory cytokines decreased the expression of the RLBP1 protein. Differentiated ARPE-19 cells were treated with the cytokines (1X = interferon gamma (IFN-γ), 10 u/ml + tumor necrosis factor alpha (TNF-α), 1 ng/ml + interleukin-1 beta (IL-1β), 1 ng/ml) for 4 days in the presence of 1% serum. A: Western immunoblot analysis of RLBP1 expression. Lane 1: molecular weight markers; lane 2: control; lane 3: cytokines, 1X; and lane 4: cytokines, 10X. A representative blot from three similar experiments is shown. B: Histogram showing RLBP1 expression. The RLBP1 band intensity was normalized with corresponding α-tubulin band intensity; *p<0.05 when compared to control, n = 3.