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GHSR1a shRNA upregulated PTEN and inhibited the PI3K/AKT pathway in SW480 cells. (A) Western blot analysis of PTEN, p‐AKT, and p53 after transducted with culture medium (BC), SC, and GHSR1a shRNA (KD). (B) The PTEN mRNA was detected by RT‐PCR assay. Data were derived from three experiments with six replicates. *P < 0.05 versus SC group. shRNA, short hairpin RNA; BC, blank control; SC, scrambled control; KD, knockdown.
