Figure 3.

The effect of silencing MARCH1 on cell proliferation, migration, and invasion in vitro. Cell proliferation was examined by CCK-8 assay: (A) silencing MARCH1 resulted in significant growth-inhibition from day 2. EdU-based proliferation assay was performed 48 h after RNA interference. At the proliferation stage the nuclei were dyed red with EdU. All nuclei were dyed blue with Hoechst. (B) Red and blue were merged into magenta to show the proportion of nuclei with proliferation. Proliferation of MARCH1-silenced cells was clearly inhibited. Downregulation of MARCH1 resulted in a decrease in (C) cell-migrated and (D) -invaded cells; ^*p<0.05 vs. the NC.