Figure 5.

Silencing MARCH1 inhibits the NF-κB pathway. (A) NF-κB luciferase reporter results indicated that silencing MARCH1 downregulated the activity of the NF-κB pathway. Silencing MARCH1 inhibited p50 and p65 expression at the (B) nucleus protein level and (C) attenuated translocation of p50 and p65. Inhibiting the NF-κB pathway by PDTC. MARCH1 expression was decreased at (D) mRNA and (E) protein levels. (F) Silencing p50 and p65 by siRNA reduced MARCH1 expression at both mRNA and (G) protein level; ^*p<0.05 vs. the NC or mock group.