Figure 3.

The effect of PPM1D silencing combined with TMZ on apoptosis of U87-MG cells. The number of apoptotic cells was determined on day 4 after TMZ treatment using flow cytometry. (A) CON group, (B) NC group, (C) KD group. (D) Summary of the experiment illustrated in (A–C). (E) To exactly analyze the influences induced by PPM1D silencing, cells in NC and KD groups with positive expression of GFP were analyzed. Apoptotic cells significantly increased in KD group with PPM1D silencing when treated with TMZ. Error bars indicate ± SD of three replicates. *P<0.05 vs. NC.