Fig 8.

Down-regulation of CMKLR1 and GPR1 A. CHO-K1 cells expressing chemerin receptors were incubated with 100 nM chemerin for various periods of time. Cell surface receptor was detected by flow cytometry using a saturating concentration of antibodies specific for CMKLR1 (●), GPR1 (◯) or CCRL2 (△). Results were normalized for the fluorescence of unstimulated cells (100%) and for background fluorescence (0%). Data represent the mean ± S.E.M. of three independent experiments. B. CHO-K1 cells expressing chemerin receptors were first incubated with ¹²⁵I-chemerin at 4°C and washed with binding buffer containing 500 mM NaCl to eliminate the unbound tracer. Then, cells were either left at 4°C or shifted to 28°C to allow receptor internalization. After 90 minutes, cells were acid-washed and the amount of radioactivity remaining associated with the cells was measured. Data represent the mean ± S.E.M. of three independent experiments.