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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2016 May 11;15(8):1943–1951. doi: 10.1158/1535-7163.MCT-15-0306

Figure 4.

Figure 4

Mechanism of how recruited T cells increase BCa cell invasion. (a) After co-culture with HH cell for 3-4 days, AR mRNA levels and (b) protein levels were measured in T24 and J82 cells. (c) ARE-luciferase assay indicated that AR activity increased after BCa cells were co-cultured with T cells. (d) T24 and J82 invasion assays were performed after knocking down AR with AR-siRNA, and then with/without co-culture with HH cells. (e) IL-1, IL-8 and AR mRNA levels of T24 cells tested after co-culture with/without IL-1 antagonist IL-1RA. (f) Invasion assay performed after treating with IL-1 antagonist. (g) AR mRNA level of T24 cells tested after co-culture and addition of 1 mg/mL IL-8 neutralizing antibody.