Fig 6. Hemopexin (Hp) is required to increase levels of p-HDL in SCD.

The lipoprotein fractions were isolated by HPLC assay to detect p-HDL (accumulation of ROS) in wild-type (WT), Hp−/− and Hx−/− mice. A significantly lower level of p-HDL is seen in SCD mice with Hp knock-out, compared with other SCD groups, confirming that Hp is the protein necessary for the proinflammatory properties of HDL. (Other = other fractions of lipoprotein; RFU, relative fluorescence units).