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. 2016 Oct 7;11(10):e0164186. doi: 10.1371/journal.pone.0164186

Fig 2. Naringin’s effect on the AMPK-mediated HO-1 expression in macrophages.

Fig 2

Cells were exposed to various concentrations of naringin for 8 h. HO-1 mRNA and protein expression were analyzed by RT-PCR and western blotting (A). Phosphorylated AMPK (p-AMPK), AMPK, phosphorylated ACC (p-ACC), and ACC expression were analyzed by western blotting with various concentrations of naringin at 90 min (B) and at 15, 30, 60, and 90 min after treatment with 200 μM naringin (C). Knockdown of AMPKα1 with siRNA treatment was confirmed by western blotting (D). HO-1 expression was measured in AMPKα1-silenced macrophages either with or without naringin treatment. *Significantly different from control.