Skip to main content
. 2016 Oct 7;11(10):e0164186. doi: 10.1371/journal.pone.0164186

Fig 4. Naringin’s effect on Nrf2 nuclear translocation and transcriptional activity.

Fig 4

Macrophages transiently transfected with ARE–luciferase reporter plasmid were treated with naringin at the indicated concentrations with or without AMPKα knockdown by siRNA or SB203508 (10 μM) and subjected to a luciferase assay (A). Macrophages were treated with naringin at the indicated concentrations for 24 h with or without AMPKα knockdown by siRNA or SB203508 (10 μM). Nuclear extracts were then subjected to western blotting to determine Nrf-2 expression levels (B). *Significantly different from control. #Significantly different from cells treated with naringin + LPS.