Skip to main content
. 2016 Oct 7;11(10):e0163697. doi: 10.1371/journal.pone.0163697

Fig 2. Expression in E. coli Origami B (DE3), purification and removal of the thioredoxin fusion part (Trx) of α2-PI1-8-VEGF121.

Fig 2

Molecular weight markers (1), uninduced cells (2), cells induced with IPTG and L-arabinose, soluble fraction (3), cells induced with IPTG and L-arabinose, insoluble fraction (4), unbound proteins (5), purified fusion protein Trx-α2-PI1-8-VEGF121 (6), preparation of the fusion protein Trx-α2-PI1-8-VEGF121 after cleavage with thrombin (7), purified α2-PI1-8-VEGF121 (8).