Fig 5. The effect of thrombin (0.01, 0.05, 0.1 and 1.0 NIH U/mL) on the proliferation of HUVEC cells.

Seeding density 3 500 cells per well of a 96-well E-plate in EBM-2 basal medium supplemented with ascorbic acid, hydrocortisone, heparin, gentamicin, amphotericin-B and 2% fetal bovine serum from EGM-2 SingleQuots and in the presence of the commercial VEGF₁₂₁ I standard (50 ng/mL) or α₂-PI_1-8-VEGF₁₂₁ followed by the xCELLigence system.