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. 2016 Oct 7;12(10):e1005924. doi: 10.1371/journal.ppat.1005924

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© 2016 Miles et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — Disaggregated cells from lymph nodes and spleen of SIV-uninfected (n = 6) and SIV-infected (n = 6) rhesus macaques were analyzed for CD8 T_FR by flow cytometry. (A) Flow gating strategy to determine viable CD8 T_FR (CD3+CD8+CXCR5^hiCD44^hi) and CD8 conv. (B) Percent CD8 T_FR in SIV-uninfected compared to SIV-infected rhesus macaques. (C) Percent of SIV-Gag tetramer+ CD8 T_FR compared to CD8 conv. CD8 T_FR and CD8 conv from SIV-uninfected and SIV-infected rhesus macaques were analyzed for percent or MFI expression of (D) Tim-3, (E) perforin, (F) IL-10, and (G) GITR. Graphs depict median and range. Statistical significance was determined by non-parametric one-way ANOVA (Friedman test) and is displayed as * = p<0.05 and *** = p<0.001.