Fig 9. Stear-MST2 disruptor peptide blocks MST2 interactions with (A) RASSF1A, and (B) with other MST2.

(A) MCF7 cells were co-transfected with Myc-tagged and Flag-tagged MST2. 48 h after transfection, cells were incubated with 10 μM of N-terminal stearoylated peptides (stear-MST2 or stear-scrambled as control) for 1 h. The cells were lysed, and MST2 was immunoprecipitated (IP) with antibody against the Flag-tag and Western blotted (WB) with anti-Myc tag antibody in order to detect MST2 homodimers. The experiment was also repeated in a second way using an anti-Myc tag antibody for IP and anti-Flag for Western blotting. (B) The cells lines indicated here were incubated with 10 μM of N-terminal stearoylated peptides (stear-MST2 or stear-scrambled as control) for 1 h. The cells were lysed, and endogenous MST2 was IP and WB for associated RASSF1A. As MCF7 cells do not express endogenous RASSF1A, they were transfected with an HA-tagged RASSF1A expression vector.