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. 2016 Oct 7;11(10):e0164215. doi: 10.1371/journal.pone.0164215

Fig 6. Omeprazole blocks AA-induced cell injury by upregulating CYP 1A1.

Fig 6

(A) OM induced CYP 1A1 in HKCs. (B) HKCs cells were treated with or without 90 μM OM for 18 h before the addition of 20 μM AA-Na for 48 h. Cell viability was then detected using an MTT assay. **P≤0.01 vs. HKCs; ## P≤0.01 vs. HKCs after 20 μM AA-Na administration. (C-D) HKCs were stably transfected with PCDNA6-CYP 1A1 (HKC-CYP 1A1) or PCDNA6 (HKC-V; control), and CYP 1A1 was ectopically expressed at the (C) mRNA and (D) protein levels in the targeted cell line. (E) HKC-V and HKC-CYP 1A1 cells were treated with or without 20 μM AA-Na or solvent control for 48 h. Cell viability was detected using an MTT assay. **P≤0.01 vs. HKC-V cells; #P≤0.05 vs. HKC-V cells treated with AA-Na. OM, omeprazole; AA-Na, aristolochic acid sodium salt. Data are expressed as mean ± SEM.