Reagent or resource	Source	Identifier
Antibodies
Mouse monoclonal anti-vimentin	Sigma-Aldrich	V6630
Rabbit anti-Vimentin against a C-terminal peptide	Santa Cruz	SC7557
Mouse anti-α-tubulin	Genetex	GT114
Rabbit anti-α-tubulin	Abcam	ab18251
Mouse anti-GFP	Roche	11 814 460 001
Mouse anti-RFP	Thermo Fisher	RF5R
Mouse anti-GAPDH	Genetex	GT239
Mouse anti-β-actin	Sigma-Aldrich	AC-15
Rabbit anti-GAPDH	Genetex	GTX100118
Rabbit anti γ-tubulin	Abcam	ab16504
Rabbit anti-GM130	Abcam	ab52649
Rabbit anti paxillin antibody	Abcam	ab32084
Mouse anti tubulin antibody	Genetex	GTX628802
Chemicals
Nocodazole	Sigma-Aldrich	M1404
Fibronectin	Sigma-Aldrich	F1141
Experimental Models: Cell Lines
hTERT-RPE-1	ATCC	CRL-4000
hTERT-RPE-1 mEmerald-vimentin	This paper	C035_2G2
hTERT-RPE-1 mEmerald-vimentin/mTagRFPt-α-tubulin	This paper	C043_1B7
MCF-7	ATCC	HTB-22
Recombinant DNA
pRS mEmerald-VIM	This paper	Addgene: #62849
pRS pRS-mTagRFP-TUBA1B	This paper	Addgene: #62850
pGoldyTALEN-VIM-TAL-1	This paper	Addgene: #62855
pGoldyTALEN-VIM-TAL-2	This paper	Addgene: #62852
pGoldyTALEN-TUBA1B-TAL-1	This paper	Addgene: #62853
pGoldyTALEN-TUBA1B-TAL-2	This paper	Addgene: #62854
mEmerald-vimentin plasmid	Helfand et al., 2011	N/A
pCGS652	This paper	Addgene: #62220
Golden Gate TALEN and TAL Effector Kit 2.0	Cermak et al. 2011	Addgene: Kit # 1000000024
BAC VIM	BACPAC Resources Center at Children’s Hospital Oakland Research Institute, Oakland, CA	RP11-315P19
BAC TUBA1B	BACPAC Resources Center at Children’s Hospital Oakland Research Institute, Oakland, CA	RP11-437A15
Software and Algorithms
Filament Network Analysis Software 2.0	This paper	https://data.mendeley.com/datasets/dywxyj9xyz/draft?a=06981b3a-e4d4-49a3-8a21-b637cfc5d246
Deposited data
Live cell imaging raw data for vimentin/microtubules interaction	This paper	https://data.mendeley.com/datasets/xycvj95pw9/draft?a=f373835d-be67-453e-913a-a49d33d4e6d5