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. 2016 Sep 9;5:e16996. doi: 10.7554/eLife.16996

Figure 5. hh inhibition does not ablate planarian glia.

(A) FISH of if-1/cali (magenta) and immunofluorescence of IF-1 (green) in animals following reduced RNAi treatment (fed d0, d4, d8, fixed d12) of a control gene, hh, or ptc. (B) Double FISH for if-1/cali (magenta) with gs (first row), gat (second row), eaat2-1 (third row), or eaat2-2 (fourth row) (green) following inhibition of a control gene (first column), hh (second column), or ptc (third column). (C) Stacked bar graph of the number of cells per square millimeter of cephalic ganglia inside the neuropil (left) and number of cells per square millimeter of the head outside the neuropil (right) expressing gat following inhibition of a control gene, hh, or ptc. Bar sections denote the ratio of if-1+/cali+subpopulation (white) to if-1-/cali-subpopulation (green). Statistical significance indicated by labels (n.s., not significant, ***p≤ 0.0001, two-tailed t test). Anterior up, ventral side shown for A–B. Scale bars: 100 um for A–B.

DOI: http://dx.doi.org/10.7554/eLife.16996.038

Figure 5—source data 1. Cell counts for co-expression of if-1/cali and gat following Hh pathway perturbation.
DOI: http://dx.doi.org/10.7554/eLife.16996.039
elife-16996-fig5-data1.xlsx (51KB, xlsx)
DOI: 10.7554/eLife.16996.039

Figure 5.

Figure 5—figure supplement 1. Glial marker expression levels in RNA-seq datasets.

Figure 5—figure supplement 1.

Comparison of gene expression levels from cephalic ganglia tissue samples following inhibition of hh or ptc. Differential expression magnitudes are statistically significant for if-1 and cali only (*padj≤0.05, **padj≤0.01).
DOI: http://dx.doi.org/10.7554/eLife.16996.040
Figure 5—figure supplement 2. Expression of glial markers in anterior blastemas.

Figure 5—figure supplement 2.

Double FISH for if-1 and cali (magenta) and gat (green) or triple FISH for if-1 and cali (magenta), eaat2-1 (blue), and eaat2-2 (green) in d6 anterior blastemas of trunks following inhibition of a control gene (left column), hh (middle column), or ptc (right column). White dotted line delineates edge of animal. Yellow dotted line delineates approximate amputation plane. Anterior up, ventral side shown. Scale bars: 100 um.
DOI: http://dx.doi.org/10.7554/eLife.16996.041