Figure 3. GFP-Q localization requires GerM and AH.
A. Representative images of GFP-Q localization in sporulating cells at hour 2 of sporulation. Images are from wild-type (BCR46), ΔAH (BCR56), ΔgerM (BCR1211), the ΔAH ΔgerM double mutant (BCR1197), and ΔsigE (BKM1930). Scale bar represents 2 μm. B. Representative images of GFP-QQ168A localization in sporulating cells at hour 2 of sporulation. Images show GFP-QQ168A in an otherwise wild-type background (BCR87), ΔAH (BCR80), or ΔgerM (BCR1313). C. Bar graph showing sporulation efficiencies of wild-type (BCR163), QQ168A (BCR152), ΔgerM (BCR1314), the ΔgerM QQ168A double mutant (BCR1313), ΔAH (BCR1335), the ΔAH QQ168A double mutant (BCR1334) and ΔQ (BTD1541). Error bars represent standard deviations (n=2).