Figure 6. GerM is sufficient to localize GFP-Q at the sporulation septum.

A. Experimental rationale and schematic outcomes for GFP-Q localization (in green) when SpoIID, SpoIIP and SpoIIM, alone (BCR1444) or together with either GerM (BCR1447) or AH (BCR1446) are artificially produced in a ΔsigE mutant. B. Representative images of cells at hour 2.5 of sporulation in which IPTG (1 mM final) was added 1.5 hours after the onset of sporulation. Examples of engulfing septal membranes with enrichment of GFP-Q in the forespore membranes are indicated (yellow carets). Scale bar represents 2 μm. As described previously (Rodrigues et al., 2013), in a subset of sporulating cells (in strain BCR1444 and derivatives) in which IPTG was added GFP-Q lost compartmentalization (not shown). Images of the same strains from the same time point in the absence of IPTG can be found in Figure S8. C. Larger images highlighting GFP-Q enrichment in the engulfing membrane when either GerM or AH was induced. D. Immunoblot analysis monitoring SpoIID, SpoIIP, GerM-His6 and AH accumulation upon the addition of IPTG. σF was used to control for loading.