Figure 5. MEF-secreted factors make primed hESCs less reliant on glucose for proliferation.
Proliferation rates of primed feeder-free H1 (A) and H9 (B) hESCs treated with DMSO, AZD3965 (MCT1i), MEF-conditioned medium (CM), or AZD3965 along with MEF-conditioned medium (CM + MCT1i) for 5 days. Glucose consumption and lactate production rates of feeder-free H1 (C) and H9 (D) primed hESCs FF, treated with DMSO, AZD3965 (MCT1i), MEF conditioned medium (CM), or AZD3965 along with MEF conditioned medium (CM + MCT1i) for 5 days. For (A) – (D), error bars indicate ± 1 SEM of biological replicates (n = 3). ns: not significant; * p < 0.05; ** p < 0.01; *** p < 0.001. See also Figure S5.