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. Author manuscript; available in PMC: 2017 Oct 6.
Published in final edited form as: Cell Stem Cell. 2016 Sep 8;19(4):476–490. doi: 10.1016/j.stem.2016.08.008

Figure 6. MYC activity in hESCs is modulated by MEF-secreted factors.

Figure 6

(A) GSEA mountain plots displaying enrichment of MYC-regulated gene sets in feeder-free (FF) versus feeder-supported (FS) primed H1, H9, and HSF1 hESCs. (B) GSEA mountain plots displaying enrichment of MYC-regulated gene sets in feeder-free (FF) versus MEF-conditioned medium treated FF (CM) primed H9 and HSF1 hESCs. Immunoblot showing nuclear and cytoplasmic N-MYC and C-MYC levels in FS, FF and MEF-conditioned medium (CM) treated primed H9 hESCs (C) and HSF1 hESCs (D). For (C) and (D), the heatmap shows standardized levels of indicated markers across samples (Z score). TBP was used to control for nuclear lysate loading, and GAPDH was used to control for cytoplasmic lysate loading. Lysates were prepared in biological duplicates.