Table 4.

Validation data of the analytical methodology

Element	Linearity
	Calibration curve range (μg/mL)	Calibration curve	R 2
Ca K Mg Na P Mn Fe Zn Cu Co Cd Cr Ni Pb	2.00–15.0 0.50–1.50 0.30–0.90 0.50–1.20 0.10–1.20 0.15–5.00 1.00–10.0 0.20–1.00 0.50–4.00 1.00–5.00 0.20–2.00 0.20–2.00 0.50–2.00 0.20–2.00	y = 0.06587x + 0.0198 y = 0.00041x + 0.0052 y = 0.00131x + 0.0236 y = 0.00065x + 0.0120 y = 0.00444x + 0.0117 y = 0.00015x + 0.0067 y = 0.05768x + 0.0065 y = 0.00037x + 0.0040 y = 0.00011x + 0.0049 y = 0.00006x + 0.0053 y = 0.00021x + 0.0067 y = 0.00006x + 0.0013 y = 0.00006x + 0.0008 y = 0.00005x + 0.0001	0.999 0.999 0.998 0.998 0.999 0.999 0.998 0.999 0.999 0.999 0.998 0.999 0.999 0.999